Summary
Published in Plant Cell, Tissue and Organ Culture 27: 95-103
Different carbohydrates were investigated for somatic embryo development of black spruce and red spruce. They were tested in a basal maturation medium consisting of Litvay's salts at half-strength containing 1 g l-1 glutamine, 1 g l-1 casein hydrolysate, 7.5 mM abscisic acid, and 0.9% Difco Bacto-agar. A comparison of different sucrose concentrations showed that 6% was optimal for embryo development. Among the nine carbohydrates tested, sucrose, fructose, glucose, maltose, and cellobiose supported embryo development while arabinose, mannitol, myo-inositol, and sorbitol did not. A comparison of sucrose, glucose, and fructose at three concentrations showed that the general pattern of response for both species followed concentration expressed as a percentage, independent of the molarity of carbohydrate in the medium. Interspecific differences were observed concerning carbohydrate requirements. For red spruce, 6% fructose was found best for embryo development, while no such preference was observed for black spruce. No significant difference was observed in the number of embryos produced with 6% sucrose or 3% sucrose plus an equimolar concentration of either mannitol, sorbitol, or myo-inositol in the maturation medium, suggesting that the effect of the carbohydrate on the maturation was partly osmotic.
Sector(s):
Forests
Categorie(s):
Scientific Article
Theme(s):
Forestry Research, Forests, Seeds and Forest Seedlings Production
Author(s)
TREMBLAY, Laurence and Francine M. TREMBLAY
Year of publication :
1991
How to get the publication :
Keywords :
maturation, somatic embryogenesis, carbohydrates, somatic embryos, development, black spruce, red spruce, seeds and forest seedlings production, Picea mariana, Picea rubens, production de semences et de plants, développement, embryogenèse somatique, carbohydrate, embryons somatiques, épinette noire, épinette rouge, production de plants
