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RAPDs as an aid to evaluate the genetic integrity of somatic embryogenesis-derived populations of Picea mariana (Mill.) B.S.P.

Summary

Published in Theoretical and Applied Genetics 86: 81-87

The usefulness of random amplified polymorphic DNA (RAPD) in assessing the genetic stability of somatic embryogenesis-derived populations of black spruce [Picea mariana (Mill.) B.S.P.] was evaluated. Three arbitrary 11-mer primers were successfully used to amplify DNA from both in-vivo and in-vitro material. Twenty-five embryogenic cell lines, additional zygotic embryos and megagametophytes from three controlled crosses involving four selected genotypes of black spruce were used for the segregation analysis of RAPD variants. Ten markers were genetically characterized and used to evaluate the genetic stability of somatic embryos derived from three embryogenic cell lines (one cell line per cross, 30 somatic embryos per cell line). No variation was detected within clones. The utilization of RAPD markers both for the assessment of genetic stability of clonal materials and to certify genetic stability throughout the process of somatic embryogenesis is discussed.

Sector(s): 

Forests

Categorie(s): 

Scientific Article

Theme(s): 

Forestry Research, Forests, Seeds and Forest Seedlings Production

Author(s)

ISABEL, Nathalie, Laurence TREMBLAY, M. MICHAUD, Francine M. TREMBLAY and Jean BOUSQUET

Year of publication :

1993

Format :

Paper

Keywords :

genetic markers, genetic stability, somatic embryogenesis, black spruce, seeds and forest seedlings production, Picea mariana, forestry research scientific article

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